US

Whip your experiments into shape with the toughest mix around

 

Experience the Amplification Difference. ToughMix®.

Quantabio ToughMix chemistry is the trade secret difference to our mastermix formulations. It has helped scientists for more than 20 years to amplify and analyze challenging samples by overcoming common PCR inhibitors. Widely used across a multitude of molecular biology applications, ToughMix formulations can be used directly from crude lysates as well as following an extraction protocol.

 

What can ToughMix do for you

  • Work directly with crude lysates
  • Avoid expensive and time-consuming purification steps
  • Compatible with a wide range of probe designs and detection chemistries
  • High quality Taq polymerase – free of residual host DNA
  • Neutralize problem causing inhibitors present in crude samples

 

 

 

Inhibitors Common Source Reagent performance
    Competitor ToughMix
Polyphenols Plant extracts
Humic acid Soil
Plant tissues
Hematin Dried bloods
Blood spots
Hemoglobin Blood
Polysaccharides Feces
Plant tissues
Melanin Hair
Skin
Engineered Application Specific Enzymes
Optimized Tough Reagent
Superior Product Performance

ToughMix technology is available for different applications:

Conventional PCR
Features
Concentration
2x
2x
Kit Format
1 tube
1 tube mix + 1 tube loading dye
Amplicon Length
Up to 24kb
Up to 4kb
Applications
High fidelity PCR, Crude PCR, Fast PCR, Long PCR, High Yield, NGS
Routine PCR, Crude PCR
Available Formats
Master Mix
Packaging (Rxns/Units)
25 Rxns
100 Rxns
500 Rxns
100 Rxns
800 Rxns
4000 Rxns

Product Finder

Select Your Assay

Starting Template

Assay Format

Detection Chemistry

Multiplexing (more than 3 targets)

Is gene-specific priming (GSP) required?

What current Reverse Transcriptase or cDNA kit are you using?

Select the group which contains your real-time PCR cycler

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®
  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Azure Cielo 6
  • Azure Cielo 3
  • Other
  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Choose your application from the categories below

Products

I give Quantabio or an authorized Quantabio distributor permission to contact me for product updates and news.
* Required information