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Product Specification Form (PSF) / Certificate of Analysis (CofA)

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Products
Sample Preparation
Extracta DNA Prep
5PRIME Phase Lock Gel
Extracta DBS
Reverse Transcription
First-Strand cDNA Synthesis
qScript cDNA SuperMix
qScript XLT cDNA SuperMix
qScript cDNA Synthesis Kit
qScript Flex cDNA Kit
PerfeCTa DNase I
Reverse Transcription PCR (RT-PCR)
Conventional RT-PCR
qScript XLT 1-Step RT-PCR Kit
Quantitative RT-qPCR
qScript XLT 1-Step RT-qPCR ToughMix
qScript One-Step SYBR Green RT-qPCR
UltraPlex 1-Step ToughMix
qScript One-Step RT-qPCR Kit
PCR & qPCR
Real-Time Quantitative PCR
SYBR Green Detection
PerfeCTa SYBR® Green SuperMix
PerfeCTa SYBR® Green FastMix
Probe-based Detection
PerfeCTa MultiPlex qPCR SuperMix
PerfeCTa qPCR ToughMix
PerfeCTa Multiplex qPCR ToughMix
PerfeCTa FastMix II
PerfeCTa qPCR FastMix UNG
Multiplexed Pre-Amplification
PerfeCTa PreAmp SuperMix
Conventional PCR
AccuStart Taq DNA Polymerase HiFi
AccuStart II PCR SuperMix
AccuStart II Taq DNA Polymerase
AccuStart II PCR ToughMix
5PRIME HotMaster Taq DNA Polymerase
5PRIME HotMasterMix
AccuStart II GelTrack PCR SuperMix
Cloning
repliQa™ HiFi Assembly Mix
Genotyping
AccuStart II PCR Genotyping Kit
AccuStart Genotyping ToughMix
AccuMelt HRM SuperMix
microRNA Profiling
qScript microRNA cDNA Synthesis Kit
PerfeCTa SYBR® Green SuperMix
Next Generation Sequencing (NGS)
PerfeCTa NGS Quantification Kit
sparQ HiFi PCR Master Mix
sparQ DNA Library Prep Kit
sparQ DNA Frag & Library Prep Kit
Materials
Website Content
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Product Manuals
Safety Data Sheets (SDS)
CofA (PSF)
Publications
FAQs
Product Flyers
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Technical Notes
FAQs
  • PCR & qPCR
    Real-Time Quantitative PCR
    SYBR Green Detection
    What is the amount of cDNA used per TaqMan or SYBR qPCR Assay?
    Suggested input quantities of template are: cDNA corresponding to 1 pg to 100 ng of total RNA; 100 pg to 100 ng genomic DNA. For more information, please consult the PerfeCta qPCR Supermixes or PerfeCta SYBR Green Supermixes protocols.
    Is there a SYBR® Green Fast Master Mix currently available
    Yes. PerfeCTa SYBR Green Supermix is available. For further information, please consult the Kit protocol or product information.
  • microRNA Profiling
    What is the amount of cDNA used per TaqMan or SYBR qPCR Assay?
    Suggested input quantities of template are: cDNA corresponding to 1 pg to 100 ng of total RNA; 100 pg to 100 ng genomic DNA. For more information, please consult the PerfeCta qPCR Supermixes or PerfeCta SYBR Green Supermixes protocols.
    Is there a SYBR® Green Fast Master Mix currently available
    Yes. PerfeCTa SYBR Green Supermix is available. For further information, please consult the Kit protocol or product information.
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