Quantabio Resource Center

Download the most current documents for your Quantabio product. Use the search bar for a simple keyword search or apply product and material filters to limit search results.

Product Specification Form (PSF) / Certificate of Analysis (CofA)

CofAs are included in our Product Specification form (PSF). Many CofAs are availabe on demand. Enter your Lot# to the search bar to find your document. Contact customerservice@quantabio.com in case your CofA is not available yet.

Filter
Products
Sample Preparation
Extracta DNA Prep
5PRIME Phase Lock Gel
Extracta DBS
Reverse Transcription
First-Strand cDNA Synthesis
qScript cDNA SuperMix
qScript XLT cDNA SuperMix
qScript cDNA Synthesis Kit
qScript Flex cDNA Kit
PerfeCTa DNase I
Reverse Transcription PCR (RT-PCR)
Conventional RT-PCR
qScript XLT 1-Step RT-PCR Kit
Quantitative RT-qPCR
qScript XLT 1-Step RT-qPCR ToughMix
qScript One-Step SYBR Green RT-qPCR
UltraPlex 1-Step ToughMix
qScript One-Step RT-qPCR Kit
PCR & qPCR
Real-Time Quantitative PCR
SYBR Green Detection
PerfeCTa SYBR® Green SuperMix
PerfeCTa SYBR® Green FastMix
Probe-based Detection
PerfeCTa MultiPlex qPCR SuperMix
PerfeCTa qPCR ToughMix
PerfeCTa Multiplex qPCR ToughMix
PerfeCTa FastMix II
PerfeCTa qPCR FastMix UNG
Multiplexed Pre-Amplification
PerfeCTa PreAmp SuperMix
Conventional PCR
AccuStart Taq DNA Polymerase HiFi
AccuStart II PCR SuperMix
AccuStart II Taq DNA Polymerase
AccuStart II PCR ToughMix
5PRIME HotMaster Taq DNA Polymerase
5PRIME HotMasterMix
AccuStart II GelTrack PCR SuperMix
Cloning
repliQa™ HiFi Assembly Mix
Genotyping
AccuStart II PCR Genotyping Kit
AccuStart Genotyping ToughMix
AccuMelt HRM SuperMix
microRNA Profiling
qScript microRNA cDNA Synthesis Kit
PerfeCTa SYBR® Green SuperMix
Next Generation Sequencing (NGS)
PerfeCTa NGS Quantification Kit
sparQ HiFi PCR Master Mix
sparQ DNA Library Prep Kit
sparQ DNA Frag & Library Prep Kit
Materials
Website Content
Products
Product Manuals
Safety Data Sheets (SDS)
CofA (PSF)
Publications
FAQs
Product Flyers
Brochures
Technical Notes
FAQs
  • Sample Preparation
    What is the shelf life of the Extracta DBS product?
    The shelf life is 2 years stored at 2-8°C.
    What is the Extracta DBS kit and what are some of the advantages of using this kit?
    The Extracta DBS kit is a research use only reagent for rapid isolation of DNA from dried blood spots. It is supplied as a convenient “ready to use” solution that reduces sample preparation time and is compatible with automation. The numerous wash and/or proteolytic digestion steps of other protocols are not necessary with Extracta DBS and the eluted DNA solution does not interfere with downstream DNA amplification applications. Qiagen Beverly’s ISO13485 manufacturing process ensures consistent composition and performance across lots.
    Should all of the supernatant be removed after the first wash and centrifugation step?
    After the first wash and centrifugation step, remove and discard as much liquid and debris as possible. The DNA remains on the filter paper at this point.
    What Quantabio products have confirmed compatibility with DNA extracted by the Extracta DBS kit?
    The following Quantabio products have been demonstrated to perform well with the extracted DNA: Accustart Genotyping ToughMix (95115, 95116, 95117) PerfeCTa Multiplex qPCR ToughMix (95147, 95148, 95149) PerfeCTa qPCR ToughMix (95112, 95113, 95114, 95138, 95139, 95140)
    For what downstream applications can the extracted DNA be used?
    The extracted DNA is compatible with a variety of end-point PCR, real-time PCR, next generation sequencing, or Sanger sequencing procedures.
    Can the extracted DNA be stored?
    We recommend using the DNA in downstream applications immediately after extraction. If this is not possible, the extracted DNA can be refrigerated at 2-8°C for short term storage (1-2 days) or stored frozen at -15 to -25°C for prolonged storage. We have no data on the stability of extracted DNA after prolonged storage.
    What is the stability of the extracted DNA?
    We have no data on the stability of the extracted DNA. We recommend that the DNA be used for downstream applications as soon as possible following extraction.
    How should the extracted DNA be measured?
    Measurement of DNA by methods using DNA binding dyes or absorbance at 260 nm are not applicable to a crude lysis method such as the Extracta DBS method. The recommended way to accurately quantify DNA in an Extracta DBS lysate is to measure a specific DNA sequence (generally a single-copy gene in gDNA) by qPCR.
There are no results for your search, click here to reset your search.