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qScript Ultra SuperMix

Faster, better, tougher next generation cDNA SuperMix for first strand cDNA synthesis
Features & Benefits
  • Enhanced Thermostability – Engineered for RT activity up to 65°C, overcoming challenging secondary structures
  • Superior Speed – 10 minute reaction time with a total protocol time of 13 minutes
  • Maximum Yield & Sensitivity – Wide dynamic range with no loss in cDNA abundance linearity from total RNA 2.5 µg – 1 pg
  • Ultimate Inhibitor Resistance – Overcome a wide array of PCR inhibitors (salt, heparin, melanin, etc.)
  • Balanced Coverage of Long Transcripts – Unbiased representation of the transcriptome in cDNA product
  • Ease of Use – Single tube mix with all required components for cDNA synthesis except RNA template

 

qScript Ultra SuperMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

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Product
Kit Size
Order Info
Price
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qScript Ultra SuperMix

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Kit Size:  25 x 20 µL rxns
Part Number:  95217-025
Price:  $184.00
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Kit Size:  100 x 20 µL rxns
Part Number:  95217-100
Price:  $566.00
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Kit Size:  500 x 20 µL rxns
Part Number:  95217-500
Price:  $2,414.00
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Description

qScript Ultra SuperMix is a highly stabilized, efficient and easy-to-use single tube master mix for the synthesis of first-strand cDNA to reverse transcribe RNA to cDNA. A key component is a novel, state-of-the-art, RNase H deficient reverse transcriptase that was engineered for improved thermostability, velocity, processivity, and resistance to many common inhibitors. qScript Ultra SuperMix contains all required components for first-strand cDNA synthesis except RNA template and is directly compatible with downstream 2-step RT-qPCR or RT-PCR procedures.

Details

Details

Contents

 

95217-025

95217-100

95217-500

5X qScript Ultra SuperMix

1 x 100 µl

1 x 400 µl

1 x 2 mL

 

Optimized master mix containing buffer, magnesium, dNTPs, optimized primer mix and qScript Ultra RT

Customer Testimonials

Customer Testimonials

qScript Ultra SuperMix
"Great reagent. Not only greatly improved sensitivity for low abundance mRNAs (2 cTs lower for same input RNA and primers) and single easy to use premixed reagent, but astonishingly quick thermocycle protocol."
Fellow | University of Surrey
qScript Ultra SuperMix
"With the use of qScript Ultra SuperMix, we were able to increase cDNA input without inhibitory effects on qPCR output. Therefore, sensitivity was enhanced"
Fellow | Wageningen University & Research
qScript Ultra SuperMix
"So fast and easy to use. Would definitely recommend to people needing a cDNA supermix reagent."
Fellow | National Jewish Health
qScript Ultra SuperMix
"It was easier to use the qScript Ultra SuperMix compared to our previous cDNA synthesis kit due to there being only one reagent. It also meant we could use more of our RNA sample in the reaction, which is useful for samples with low RNA concentrations."
Scientist | Neuro-bio
qScript Ultra SuperMix
"Amazing product! Perfect stability and reliability of the result. Easy-one-step protocol makes procedure much faster and error-less."
Fellow | University of Rochester
qScript Ultra Supermix - UToronto

"The qScript Ultra Supermix worked 3x faster. More importantly, my PCR was working and I saw amplification in samples I previously saw none. The inhibitor tolerance is key for this."

Anthony Eiliazadeh | University of Toronto

Details

Contents

 

95217-025

95217-100

95217-500

5X qScript Ultra SuperMix

1 x 100 µl

1 x 400 µl

1 x 2 mL

 

Optimized master mix containing buffer, magnesium, dNTPs, optimized primer mix and qScript Ultra RT

Performance Data

Resources

Customer Testimonials

qScript Ultra SuperMix
"Great reagent. Not only greatly improved sensitivity for low abundance mRNAs (2 cTs lower for same input RNA and primers) and single easy to use premixed reagent, but astonishingly quick thermocycle protocol."
Fellow | University of Surrey
qScript Ultra SuperMix
"With the use of qScript Ultra SuperMix, we were able to increase cDNA input without inhibitory effects on qPCR output. Therefore, sensitivity was enhanced"
Fellow | Wageningen University & Research
qScript Ultra SuperMix
"So fast and easy to use. Would definitely recommend to people needing a cDNA supermix reagent."
Fellow | National Jewish Health
qScript Ultra SuperMix
"It was easier to use the qScript Ultra SuperMix compared to our previous cDNA synthesis kit due to there being only one reagent. It also meant we could use more of our RNA sample in the reaction, which is useful for samples with low RNA concentrations."
Scientist | Neuro-bio
qScript Ultra SuperMix
"Amazing product! Perfect stability and reliability of the result. Easy-one-step protocol makes procedure much faster and error-less."
Fellow | University of Rochester
qScript Ultra Supermix - UToronto

"The qScript Ultra Supermix worked 3x faster. More importantly, my PCR was working and I saw amplification in samples I previously saw none. The inhibitor tolerance is key for this."

Anthony Eiliazadeh | University of Toronto

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