Real-Time QPCR Reagents

PerfeCTa qPCR reagents combine a stringent, ultrapure antibody hotstart with performance engineered DNA polymerase in stabilized 1-tube formulations optimized for the specific performance needs of real-time quantitative PCR.  Proprietary additives help eliminate persistent bubbles to enable efficient vortex mixing and fewer technical replicates thereby conserving precious sample. Adaptive buffer chemistry accommodates most assay designs and can be used with existing assay designs.

PerfeCTa qPCR ToughMix reagents contain proprietary inhibitor-neutralizing additives that relieve several types of PCR inhibition ensuring reliable qPCR assay performance with clinical, plant or environmental specimens or complex food matix.

Quantabio quantitative real-time PCR reagents are optimized to work in series with high performance qScript RT reagents (for RNA targets) and Extracta DNA Prep (for DNA targets).

Multiplexed Pre-Amplification

  • PerfeCTa PreAmp SuperMix 5X concentrated, ready-to-use reaction cocktail for unbiased, selected enrichment of target sequences from limiting amounts of starting material for downstream gene expression profiling or targeted re-sequencing.

SYBR Green Detection

  • PerfeCTa SYBR® Green FastMix User-friendly, stabilized 2X concentrated reaction cocktail with proprietary AccuFast DNA polymerase technology for highly sensitive and specific SYBR Green based detection with either fast or standard thermal cycling conditions
  • PerfeCTa SYBR® Green SuperMix User-friendly, stabilized 2X concentrated reaction cocktail optimized to produce maximum optical signal from especially small qPCR amplicons. Not recommended for fast thermal cycling conditions (anneal/elongation step <30 seconds)

Probe-based Detection

  • PerfeCTa Multiplex qPCR ToughMix

    An advanced 5X concentrated ToughMix reagent optimized to support highly-multiplexed real-time qPCR amplification across a range of inhibitory sample types and crude extraction methods

  • PerfeCTa qPCR ToughMix A 2X concentrated ready-to-use reaction cocktail for quantitative DNA detection with crude extracts or inhibitory starting materials such as clinical or environmental specimens, plant tissues, animal tissues, and complex food matrices
  • PerfeCTa qPCR FastMix UNG Fast qPCR cycling that eliiminates carry-over contamination
  • PerfeCTa FastMix II An advanced 2X concentrated qPCR SuperMix reagent optimized to support both fast and standard thermal cycling conditions with probe-based real-time quantitative PCR assays
  • PerfeCTa MultiPlex qPCR SuperMix A 2X concentrated, ready-to-use reaction cocktail containing all the necessary components for highly-multiplexed, real-time quantitative PCR - except primers, probe(s), and DNA template